An essential factor for high Mg2+ tolerance of Staphylococcus aureus

An essential factor for high Mg2+ tolerance of Staphylococcus aureus

Blog Article

Internal bacterial concentration of Mg2+, the most abundant divalent cation in living cells, is estimated to be in the single millimolar range.However, many bacteria will thrive in media with only micromolars of Mg2+, by using a range of intensely Candles studied and highly efficient import mechanisms, as well as in media with very high magnesium concentration, presumably mediated by currently unknown export mechanisms.Staphylococcus aureus has a particularly high Mg2+ tolerance for a pathogen, growing unimpaired in up to 770 mM Mg2+, and we here identify SA0657, POWDER BURN 2.0 ORANGE SLICE a key factor in this tolerance.The predicted domain structure of SA0657 is shared with a large number of proteins in bacteria, archaea and even eukarya, for example CorB from Salmonella and the human CNNM protein family.One of the shared domains, a CBS pair potentially involved in Mg2+ sensing, contains the conserved Glycine326 which we establish to be a key residue for SA0657 function.

In light of our findings, we propose the name MpfA, Magnesium Protection Factor A, for SA0657.